NUP50 Antibody

• Background:

  The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. NUP50 is a member of the FG-repeat containing nucleoporins that functions as a soluble cofactor in importin-alpha:beta-mediated nuclear protein import. NUP50 may serve as a binding site on the nuclear side of the pore complex for export receptor-cargo complexes. It interacts with multiple transport receptor proteins including p27Kip1. This interaction is required for correct intracellular transport and degradation of p27Kip1.

• NCBI Gene ID:

  10762

• Swiss Prot:

  Q9UKX7

• Accession Number:

  Q9UKX7

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This NUP50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 432-461 amino acids from the C-terminal region of human NUP50.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  50 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  NUP50

• NCBI Official Name:

  Nuclear pore complex protein Nup50

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lindsay, M.E., et al., Cell 110 (3) :349-360 (2002) .

• Background Reference 02:

  Swaminathan, S., et al., Dev. Cell 3 (3) :304-306 (2002) .

• Background Reference 03:

  Smitherman, M., et al., Mol. Cell. Biol. 20 (15) :5631-5642 (2000) .

• Background Reference 04:

  Guan, T., et al., Mol. Cell. Biol. 20 (15) :5619-5630 (2000) .

• Other Product Names:

  Nuclear pore complex protein Nup50, 50 kDa nucleoporin, Nuclear pore-associated protein 60 kDa-like, Nucleoporin Nup50, NUP50, NPAP60L

• Tested Applications:

  WB

• Protein ID:

  20455193

• Physical Properties:

  Liquid