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PDF Antibody

CAT: 0223-60-274Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-60-274Size:400 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells.
NCBI Gene ID
64146
Swiss Prot
Q9HBH1
Accession Number
Q9HBH1
Host
Rabbit
Reactivity
Human
Immunogen
This PDF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 125-151 amino acids from the Central region of human PDF.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle, Obesity, Signal Transduction
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
27 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
PDF
NCBI Official Name
Peptide deformylase, mitochondrial
NCBI Organism
Homo sapiens
Background Reference 01
Escobar-Alvarez, S., et al. J. Mol. Biol. 387 (5) :1211-1228 (2009)
Background Reference 02
Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17 (12) :3558-3566 (2008)
Background Reference 03
Lee, M.D., et al. J. Clin. Invest. 114 (8) :1107-1116 (2004)
Background Reference 04
Serero, A., et al. J. Biol. Chem. 278 (52) :52953-52963 (2003)
Other Product Names
Peptide deformylase, mitochondrial, Polypeptide deformylase, PDF, PDF1A
Tested Applications
WB
Protein ID
17433054
Physical Properties
Liquid

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