HIST1H2BH/HIST1H2BK/HIST3H2BB Antibody

• Background:

  Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq].

• NCBI Gene ID:

  8345

• Swiss Prot:

  Q93079

• Accession Number:

  Q93079

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This HIST1H2BH/HIST1H2BK/HIST3H2BB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-52 amino acids from the Central region of human HIST1H2BH/HIST1H2BK/HIST3H2BB.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Xenopus, Mouse, Bovine, Chicken, Zebrafish, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  14 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  HIST1H2BH

• NCBI Official Name:

  Histone H2B type 1-H

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Kim, S.C., et al. Mol. Cell 23 (4) :607-618 (2006)

• Background Reference 02:

  Beck, H.C., et al. Mol. Cell Proteomics 5 (7) :1314-1325 (2006)

• Background Reference 03:

  Pavri, R., et al. Cell 125 (4) :703-717 (2006)

• Background Reference 04:

  Bonenfant, D., et al. Mol. Cell Proteomics 5 (3) :541-552 (2006)

• Other Product Names:

  Histone H2B type 1-H, Histone H2Bj, H2B/j, HIST1H2BH, H2BFJ

• Tested Applications:

  WB

• Protein ID:

  7387739

• Physical Properties:

  Liquid