GCLC Antibody

• Background :

  Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

• NCBI Gene ID :

  2729

• Swiss Prot :

  P48506

• Accession Number :

  P48506

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  This GCLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the Central region of human GCLC.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cell Cycle, Obesity, Signal Transduction

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Homology :

  Predicted species reactivity based on immunogen sequence: Rat

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  73 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  GCLC

• NCBI Official Name :

  Glutamate--cysteine ligase catalytic subunit

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Man, B.L., et al.Clin Neurosci 17 (10) :1244-1247 (2010)

• Background Reference 02 :

  Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :

• Background Reference 03 :

  Le, T.M., et al. Mol. Genet. Metab. 101 (1) :55-61 (2010)

• Background Reference 04 :

  Fullerton, J.M., et al. Bipolar Disord 12 (5) :550-556 (2010)

• Other Product Names :

  Glutamate--cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase, GCLC, GLCL, GLCLC

• Tested Applications :

  WB

• Protein ID :

  1346190

• Physical Properties :

  Liquid