SEPSECS Antibody

• Background:

  The 21st amino acid, selenocysteine (sec), is distinct from other amino acids because it lacks its own tRNA synthetase and is the only amino acid synthesized on its cognate tRNA. Synthesis of sec begins with acylation of tRNA (sec) (TRSP; MIM 165060) by seryl-tRNA synthetase (SARS; MIM 607529) to give ser-tRNA (sec), which is subsequently phosphorylated by O-phosphoseryl-tRNA kinase (PSTK; MIM 611310) to give O-phosphoseryl-tRNA (sec) . SEPSECS catalyzes the final step of sec synthesis by converting O-phosphoseryl-tRNA (sec) to selenocysteinyl-tRNA (sec) using selenophosphate as the selenium donor (Palioura et al., 2009 [PubMed 19608919]) .

• NCBI Gene ID:

  51091

• Swiss Prot:

  Q9HD40

• Accession Number:

  Q9HD40

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This SEPSECS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-474 amino acids from the C-terminal region of human SEPSECS.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  56 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  SEPSECS

• NCBI Official Name:

  O-phosphoseryl-tRNA (Sec) selenium transferase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Agamy, O., et al. Am. J. Hum. Genet. 87 (4) :538-544 (2010)

• Background Reference 02:

  Hart, K., et al. Lung Cancer (2010) In press :

• Background Reference 03:

  Volkmann, M., et al. J. Autoimmun. 34 (1) :59-65 (2010)

• Background Reference 04:

  Palioura, S., et al. Science 325 (5938) :321-325 (2009)

• Other Product Names:

  O-phosphoseryl-tRNA (Sec) selenium transferase, Liver-pancreas antigen, LP, SLA-p35, SLA/LP autoantigen, Selenocysteine synthase, Sec synthase, Selenocysteinyl-tRNA (Sec) synthase, Sep-tRNA:Sec-tRNA synthase, SepSecS, Soluble liver antigen, SLA, UGA suppressor tRNA-associated protein, tRNA (Ser/Sec) -associated antigenic protein, SEPSECS, TRNP48

• Tested Applications:

  WB

• Protein ID:

  62287911

• Physical Properties:

  Liquid