CCNF Antibody

• Background:

  This gene encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified.

• NCBI Gene ID:

  899

• Swiss Prot:

  P41002

• Accession Number:

  P41002

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This CCNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496-524 amino acids from the Central region of human CCNF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  88 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CCNF

• NCBI Official Name:

  Cyclin-F

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  D'Angiolella, V., et al. Nature 466 (7302) :138-142 (2010)

• Background Reference 02:

  Fung, T.K., et al. J. Biol. Chem. 277 (38) :35140-35149 (2002)

• Background Reference 03:

  Kong, M., et al. EMBO J. 19 (6) :1378-1388 (2000)

• Background Reference 04:

  Bai, C., et al. Cell 86 (2) :263-274 (1996)

• Other Product Names:

  Cyclin-F, F-box only protein 1, CCNF, FBX1, FBXO1

• Tested Applications:

  WB

• Protein ID:

  20178283

• Physical Properties:

  Liquid