TFE3 Antibody

• Background:

  The microphthalmia transcription factor/transcription factor E (MITF-TFE) family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors includes four family members: MITF, TFE3, TFEB and TFEC. The TEF3 protein encoded by this gene activates transcription through binding to the muE3 motif of the immunoglobulin heavy-chain enhancer. The TFEC protein forms heterodimers with the TEF3 protein and inhibits TFE3-dependent transcription activation. The TEF3 protein interacts with transcription regulators such as E2F3, SMAD3, and LEF-1, and is involved in TGF-beta-induced transcription, playing important roles in cell growth, proliferation, and osteoclast and macrophage differentiation. The TFE3 protein also activates hepatic IRS-2 gene, and induces hexokinase II (HK2) and insulin-induced gene 1 (INSIG1) ; it participates in insulin signaling and could be a therapeutic target for diabetes. This gene is also involved in chromosomal translocations, resulting in different fusion gene products in papillary renal cell carcinomas and alveolar soft part sarcomas, such as PRCC-TFE3, RCC17-TFE3, PSF-TFE3, NonO (p54nrb) -TFE3 and ASPL-TFE3.

• NCBI Gene ID:

  7030

• Swiss Prot:

  P19532

• Accession Number:

  P19532

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This TFE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 489-516 amino acids from the C-terminal region of human TFE3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  62 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  TFE3

• NCBI Official Name:

  Transcription factor E3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Argani, P., et al. Am. J. Surg. Pathol. 34 (10) :1395-1406 (2010)

• Background Reference 02:

  Haudebourg, J., et al. Cancer Genet. Cytogenet. 200 (2) :75-78 (2010)

• Background Reference 03:

  Chang, I.W., et al. Am. J. Surg. Pathol. 33 (12) :1894-1901 (2009)

• Background Reference 04:

  Yamaguchi, T., et al. Acta Cytol. 53 (6) :693-697 (2009)

• Other Product Names:

  Transcription factor E3, Class E basic helix-loop-helix protein 33, bHLHe33, TFE3, BHLHE33

• Tested Applications:

  WB

• Protein ID:

  160113240

• Physical Properties:

  Liquid