TDP1 Antibody

• Background:

  The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1) . While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform.

• NCBI Gene ID:

  55775

• Swiss Prot:

  Q9NUW8

• Accession Number:

  Q9NUW8

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This TDP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-239 amino acids from the Central region of human TDP1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  68 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  TDP1

• NCBI Official Name:

  Tyrosyl-DNA phosphodiesterase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Dexheimer, T.S., et al. Nucleic Acids Res. 38 (7) :2444-2452 (2010)

• Background Reference 02:

  Chiang, S.C., et al. Cell Cycle 9 (3) :588-595 (2010)

• Background Reference 03:

  Das, B.B., et al. EMBO J. 28 (23) :3667-3680 (2009)

• Background Reference 04:

  Zhou, T., et al. DNA Repair (Amst.) 8 (8) :901-911 (2009)

• Other Product Names:

  Tyrosyl-DNA phosphodiesterase 1, Tyr-DNA phosphodiesterase 1, 314-, TDP1

• Tested Applications:

  WB

• Protein ID:

  37999797

• Physical Properties:

  Liquid