Products for Research Use Only

CRISP1 Antibody

CAT: 0223-59-344Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-59-344Size:400 µL
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Background
Fertilization consists of a sequence of specific cell-cell interactions culminating in the fusion of the sperm and egg plasma membranes. Recognition, binding, and fusion occur through the interaction of complementary molecules that are localized to specific domains of the sperm and egg plasma membranes. In the sperm, the postacrosomal region or equatorial segment is involved in sperm-egg plasma membrane fusion. The protein encoded by this gene is a member of the cysteine-rich secretory protein (CRISP) family. This protein is expressed in the epididymis, is secreted into the epididymal lumen, and binds to the postacrosomal region of the sperm head where it plays a role at fertilization in sperm-egg fusion through complementary sites localized on the egg surface. Two isoforms are encoded by transcript variants of this gene.
NCBI Gene ID
167
Swiss Prot
P54107
Accession Number
P54107
Host
Rabbit
Reactivity
Human
Immunogen
This CRISP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162-191 amino acids from the C-terminal region of human CRISP1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cell Cycle
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
28 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
CRISP1
NCBI Official Name
Cysteine-rich secretory protein 1
NCBI Organism
Homo sapiens
Background Reference 01
Mungall, A.J., et al. Nature 425 (6960) :805-811 (2003)
Background Reference 02
Evans, J.P. Hum. Reprod. Update 8 (4) :297-311 (2002)
Background Reference 03
Cuasnicu, P.S., et al. Arch. Med. Res. 32 (6) :614-618 (2001)
Background Reference 04
Cohen, D.J., et al. Biol. Reprod. 65 (4) :1000-1005 (2001)
Other Product Names
Cysteine-rich secretory protein 1, CRISP-1, AEG-like protein, ARP, Acidic epididymal glycoprotein homolog, CRISP1, AEGL1
Tested Applications
WB
Protein ID
1706134
Physical Properties
Liquid