CRISP2 Antibody

• Background:

  CRISP2 Antibody: The cysteine-rich secretory proteins (CRISP) family is a group of four proteins that are strongly expressed in the male reproductive tract and have been implicated in having roles in male fertility. CRISP2, also known as TPX1, has been implicated in the adhesion between spermatids and Sertoli cells, and with CRISP1, is thought to be involved in sperm-egg fusion. CRISP2 has been shown to regulate the Ca2+ influx through ryanodine receptors (RYR) and may influence the acrosome reaction or sperm motility. CRISP2 has also been shown to bind to the mitogen-activated protein kinase kinase kinase 11 (MAP3K11) and localizes to the developing acrosome, suggesting this CRISP2-MAP3K11 complex may have a role in acrosome development.

• NCBI Gene ID:

  7180

• Swiss Prot:

  P16562

• Accession Number:

  NP_001135880

• Host:

  Rabbit

• Reactivity:

  Human

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  CRISP2 Antibody is affinity chromatography purified via peptide column.

• Positive Control:

  Cat. No. 1313 - Human Testis Tissue Lysate

• Concentration:

  1 mg/mL

• Buffer:

  CRISP2 Antibody is supplied in PBS containing 0.02% sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  CRISP2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Fragment:

  IgG

• Symbol:

  CRISP2

• Positive Control 2:

  Cat. No. 11-701 - Human Testis Tissue Slide

• NCBI Official Name:

  Cysteine-rich secretory protein 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Cohen DJ, Busso D, Da Ros V, et al. Participation of cysteine-rich secretory proteins (CRISP) in mammalian sperm-egg interaction. Int. J. Dev. Biol.2008; 52:737-42.

• Background Reference 02:

  Maeda T, Sakashita M, Ohba Y, et al. Molecular cloning of the rat Tpx-1 responsible for the interaction between spermatogenic and Sertoli cells. Biochem. Biophys. Res. Commun.1998; 248:140-6.

• Background Reference 03:

  Busso D, Goldweic NM, Hayashi M, et al. Evidence for the involvement of testicular protein CRISP2 in mouse sperm-egg fusion. Biol. Reprod.2007; 76:701-8.

• Background Reference 04:

  Gibbs GM, Scanlon MJ, Swarbrick J, et al. The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signaling. J. Biol. Chem.2006; 281:4156-63.

• Other Product Names:

  CRISP2 Antibody: CT36, TPX1, TSP1, GAPDL5, CRISP-2, Cysteine-rich secretory protein 2, Testis-specific protein TPX-1

• Tested Applications:

  ELISA, WB, IHC-P, IF

• Protein ID:

  215422434

• Physical Properties:

  Liquid