SPO11 Antibody

• Background:

  Meiotic recombination and chromosome segregation require the formation of double-strand breaks (DSBs) in paired chromosome homologs. During meiosis in yeast, a meiotic recombination protein is covalently-linked to the 5' end of DSBs and is essential for the formation of DSBs. The protein encoded by this gene is similar in sequence and conserved features to the yeast meiotic recombination protein. The encoded protein belongs to the TOP6A protein family. Several transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been described.

• NCBI Gene ID:

  23626

• Swiss Prot:

  Q9Y5K1

• Accession Number:

  Q9Y5K1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This SPO11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 299-326 amino acids from the C-terminal region of human SPO11.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  45 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  SPO11

• NCBI Official Name:

  Meiotic recombination protein SPO11

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Background Reference 02:

  Aston, K.I., et al. Hum. Reprod. 25 (6) :1383-1397 (2010)

• Background Reference 03:

  Mandon-Pepin, B., et al. Eur. J. Endocrinol. 158 (1) :107-115 (2008)

• Background Reference 04:

  Christensen, G.L., et al. Fertil. Steril. 84 (3) :758-760 (2005)

• Other Product Names:

  Meiotic recombination protein SPO11, Cancer/testis antigen 35, CT35, SPO11

• Tested Applications:

  WB

• Protein ID:

  7674367

• Physical Properties:

  Liquid