MTIF2 Antibody

CAT: 0223-58-834Size: 400 µLDry Ice: NoHazardous: No
CAT#:0223-58-834Size:400 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2 (mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2 (mt) shares 32 to 38% amino acid sequence identity with yeast IF-2 (mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].
NCBI Gene ID
4528
Swiss Prot
P46199
Accession Number
P46199
Host
Rabbit
Reactivity
Human
Immunogen
This MTIF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the N-terminal region of human MTIF2.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
81 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
MTIF2
NCBI Official Name
Translation initiation factor IF-2, mitochondrial
NCBI Organism
Homo sapiens
Background Reference 01
Dick, D.M., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. (2010) In press :
Background Reference 02
Reiling, E., et al. Eur. J. Hum. Genet. 17 (8) :1056-1062 (2009)
Background Reference 03
Miura, E., et al. Plant Cell 19 (4) :1313-1328 (2007)
Background Reference 04
Bouwmeester, T., et al. Nat. Cell Biol. 6 (2) :97-105 (2004)
Other Product Names
Translation initiation factor IF-2, mitochondrial, IF-2 (Mt), IF-2Mt, IF2 (mt), MTIF2
Tested Applications
WB
Protein ID
223590069
Physical Properties
Liquid

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