MTIF2 Antibody

• Background :

  During the initiation of protein biosynthesis, initiation factor-2 (IF-2) promotes the binding of the initiator tRNA to the small subunit of the ribosome in a GTP-dependent manner. Prokaryotic IF-2 is a single polypeptide, while eukaryotic cytoplasmic IF-2 (eIF-2) is a trimeric protein. Bovine liver mitochondria contain IF-2 (mt), an 85-kD monomeric protein that is equivalent to prokaryotic IF-2. The predicted 727-amino acid human protein contains a 29-amino acid presequence. Human IF-2 (mt) shares 32 to 38% amino acid sequence identity with yeast IF-2 (mt) and several prokaryotic IF-2s, with the greatest degree of conservation in the G domains of the proteins. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

• NCBI Gene ID :

  4528

• Swiss Prot :

  P46199

• Accession Number :

  P46199

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This MTIF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 138-167 amino acids from the N-terminal region of human MTIF2.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Other

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  81 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  MTIF2

• NCBI Official Name :

  Translation initiation factor IF-2, mitochondrial

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Dick, D.M., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. (2010) In press :

• Background Reference 02 :

  Reiling, E., et al. Eur. J. Hum. Genet. 17 (8) :1056-1062 (2009)

• Background Reference 03 :

  Miura, E., et al. Plant Cell 19 (4) :1313-1328 (2007)

• Background Reference 04 :

  Bouwmeester, T., et al. Nat. Cell Biol. 6 (2) :97-105 (2004)

• Other Product Names :

  Translation initiation factor IF-2, mitochondrial, IF-2 (Mt), IF-2Mt, IF2 (mt), MTIF2

• Tested Applications :

  WB

• Protein ID :

  223590069

• Physical Properties :

  Liquid