CUL2 Antibody

• Background :

  Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity) . The functional specificity of the ECS complex depends on the substrate recognition component. ECS (VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF) .

• NCBI Gene ID :

  8453

• Swiss Prot :

  Q13617

• Accession Number :

  Q13617

• Host :

  Rabbit

• Reactivity :

  Human, Mouse

• Immunogen :

  This CUL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-179 amino acids from the N-terminal region of human CUL2.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Cell Cycle

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  87 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  CUL2

• NCBI Official Name :

  Cullin-2

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Park, S.W., et al. APMIS 117 (12) :880-885 (2009)

• Background Reference 02 :

  Argyropoulos, G., et al. Physiol. Genomics 36 (2) :79-88 (2009)

• Background Reference 03 :

  Barrett, J.C., et al. Nat. Genet. 40 (8) :955-962 (2008)

• Background Reference 04 :

  Maeda, Y., et al. J. Biol. Chem. 283 (23) :16084-16092 (2008)

• Other Product Names :

  Cullin-2, CUL-2, CUL2

• Tested Applications :

  WB

• Protein ID :

  19863260

• Physical Properties :

  Liquid