CUL2 Antibody

• Background:

  Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity) . The functional specificity of the ECS complex depends on the substrate recognition component. ECS (VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF) .

• NCBI Gene ID:

  8453

• Swiss Prot:

  Q13617

• Accession Number:

  Q13617

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This CUL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-179 amino acids from the N-terminal region of human CUL2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  87 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CUL2

• NCBI Official Name:

  Cullin-2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Park, S.W., et al. APMIS 117 (12) :880-885 (2009)

• Background Reference 02:

  Argyropoulos, G., et al. Physiol. Genomics 36 (2) :79-88 (2009)

• Background Reference 03:

  Barrett, J.C., et al. Nat. Genet. 40 (8) :955-962 (2008)

• Background Reference 04:

  Maeda, Y., et al. J. Biol. Chem. 283 (23) :16084-16092 (2008)

• Other Product Names:

  Cullin-2, CUL-2, CUL2

• Tested Applications:

  WB

• Protein ID:

  19863260

• Physical Properties:

  Liquid