CUL1 Antibody

Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF (BTRC) and SCF (FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF (FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF (BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5 and probably NFKB2. SCF (SKP2) directs ubiquination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF (SKP2) directs ubiquination of ORC1L, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF (FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF (FBXW2) directs ubiquitination of GCM1. SCF (FBXO32) directs ubiquitination of MYOD1. SCF (FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF (FBXO33) directs ubiquitination of YBX1. SCF (FBXO11) does not seem to direct ubiquitination of TP53. SCF (BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF (Cyclin F) directs ubiquitination of CP110 (By similarity) .

8454

Q13616

Q13616

Human

This CUL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 680-708 amino acids from the C-terminal region of human CUL1.

Unconjugated

Primary Antibodies

Cancer, Cell Cycle

This antibody is purified through a protein A column, followed by peptide affinity purification.

Batch dependent

Predicted species reactivity based on immunogen sequence: Mouse

Supplied in PBS with 0.09% (W/V) sodium azide.

None

Blue Ice

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

90 kDa

Rabbit Ig

For WB starting dilution is: 1:1000

CUL1

Cullin-1

Homo sapiens

Gao, D., et al. Mol. Cell 39 (5) :797-808 (2010)

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

Kleiger, G., et al. Cell 139 (5) :957-968 (2009)

Cunningham, J.M., et al. Br. J. Cancer 101 (8) :1461-1468 (2009)

Cullin-1, CUL-1, CUL1

WB

19863257