RNF34 Antibody

• Background:

  The protein encoded by this gene contains a RINF finger, a motif known to be involved in protein-protein and protein-DNA interactions. This protein interacts with DNAJA3/hTid-1, which is a DnaJ protein reported to function as a modulator of apoptosis. Overexpression of this gene in Hela cells was shown to confer the resistance to TNF-alpha induced apoptosis, suggesting an anti-apoptotic function of this protein. This protein can be cleaved by caspase-3 during the induction of apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

• NCBI Gene ID:

  80196

• Swiss Prot:

  Q969K3

• Accession Number:

  Q969K3

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This RNF34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-311 amino acids from the C-terminal region of human RNF34.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  42 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  RNF34

• NCBI Official Name:

  E3 ubiquitin-protein ligase RNF34

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Erlbruch, A., et al. Proteomics 10 (16) :2890-2900 (2010)

• Background Reference 02:

  Yang, W., et al. Cell Cycle 7 (5) :670-682 (2008)

• Background Reference 03:

  Yang, W., et al. J. Biol. Chem. 282 (5) :3273-3281 (2007)

• Background Reference 04:

  Konishi, T., et al. Mol. Cancer Ther. 4 (5) :743-750 (2005)

• Other Product Names:

  HGNC:17297

• Tested Applications:

  WB

• Protein ID:

  74760679

• Physical Properties:

  Liquid