RNF14 Antibody

• Background:

  The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported.

• NCBI Gene ID:

  9604

• Swiss Prot:

  Q9UBS8

• Accession Number:

  Q9UBS8

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This RNF14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 336-364 amino acids from the C-terminal region of human RNF14.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  54 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  RNF14

• NCBI Official Name:

  E3 ubiquitin-protein ligase RNF14

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Xu, K., et al. Cancer Cell 15 (4) :270-282 (2009)

• Background Reference 02:

  Lan, K.C., et al. Fertil. Steril. 89 (5 SUPPL), 1397-1405 (2008) :

• Background Reference 03:

  Kikuchi, H., et al. Carcinogenesis 28 (8) :1752-1758 (2007)

• Background Reference 04:

  Yang, Z., et al. Endocrinology 148 (3) :1340-1349 (2007)

• Other Product Names:

  E3 ubiquitin-protein ligase RNF14, 632-, Androgen receptor-associated protein 54, HFB30, RING finger protein 14, Triad2 protein, RNF14, ARA54

• Tested Applications:

  WB

• Protein ID:

  17380293

• Physical Properties:

  Liquid