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TTL Antibody

CAT: 0223-58-579Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-58-579Size:400 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529) . Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]) .
NCBI Gene ID
150465
Swiss Prot
Q8NG68
Accession Number
Q8NG68
Host
Rabbit
Reactivity
Human
Immunogen
This TTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 232-263 amino acids from the C-terminal region of human TTL.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Pig, Rat
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
43 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
TTL
NCBI Official Name
Tubulin--tyrosine ligase
NCBI Organism
Homo sapiens
Background Reference 01
Dick, D.M., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010) :
Background Reference 02
Erck, C., et al. Cytogenet. Genome Res. 101 (1) :47-53 (2003)
Background Reference 03
Mialhe, A., et al. Cancer Res. 61 (13) :5024-5027 (2001)
Background Reference 04
Ersfeld, K., et al. J. Cell Biol. 120 (3) :725-732 (1993)
Other Product Names
Tubulin--tyrosine ligase, TTL, TTL
Tested Applications
WB
Protein ID
47117358
Physical Properties
Liquid

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