ERI1 Antibody

• Background:

  RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi) . Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

• NCBI Gene ID:

  90459

• Swiss Prot:

  Q8IV48

• Accession Number:

  Q8IV48

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This ERI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 80-109 amino acids from the N-terminal region of human ERI1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  40 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  ERI1

• NCBI Official Name:

  3'-5' exoribonuclease 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rose, J. Phd, et al. Mol. Med. (2010) In press :

• Background Reference 02:

  Mullen, T.E., et al. Genes Dev. 22 (1) :50-65 (2008)

• Background Reference 03:

  Kupsco, J.M., et al. RNA 12 (12) :2103-2117 (2006)

• Background Reference 04:

  Yang, X.C., et al. J. Biol. Chem. 281 (41) :30447-30454 (2006)

• Other Product Names:

  3'-5' exoribonuclease 1, 31--, 3'-5' exonuclease ERI1, Eri-1 homolog, Histone mRNA 3'-end-specific exoribonuclease, Histone mRNA 3'-exonuclease 1, Protein 3'hExo, HEXO, ERI1, 3'EXO, THEX1

• Tested Applications:

  WB

• Protein ID:

  45476938

• Physical Properties:

  Liquid