TOP2A Antibody

• Background :

  This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

• NCBI Gene ID :

  7153

• Swiss Prot :

  P11388

• Accession Number :

  P11388

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This TOP2A/Topo? antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1434-1461 amino acids from the C-terminal region of human TOP2A/Topo?.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  174 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  TOP2A

• NCBI Official Name :

  DNA topoisomerase 2-alpha

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Vranic, S., et al. Hum. Pathol. 41 (11) :1617-1623 (2010)

• Background Reference 02 :

  Chen, H., et al. Am. J. Surg. Pathol. 34 (9) :1250-1257 (2010)

• Background Reference 03 :

  Ye, J., et al. Cell 142 (2) :230-242 (2010)

• Background Reference 04 :

  Rossi, E., et al. Histopathology 57 (1) :81-89 (2010)

• Other Product Names :

  DNA topoisomerase 2-alpha, DNA topoisomerase II, alpha isozyme, TOP2A, TOP2

• Tested Applications :

  WB

• Protein ID :

  13959709

• Physical Properties :

  Liquid