Mouse Stk3 Antibody

• Background:

  Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1.

• NCBI Gene ID:

  56274

• Swiss Prot:

  Q9JI10

• Accession Number:

  Q9JI10

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This Mouse Stk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 387-415 amino acids from the C-terminal region of mouse Stk3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  57 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  Stk3

• NCBI Official Name:

  Serine/threonine-protein kinase 3

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Oh, H.J., et al. Curr. Biol. 20 (5) :416-422 (2010)

• Background Reference 02:

  Lu, L., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (4) :1437-1442 (2010)

• Background Reference 03:

  Song, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (4) :1431-1436 (2010)

• Background Reference 04:

  Zhou, D., et al. Cancer Cell 16 (5) :425-438 (2009)

• Other Product Names:

  Serine/threonine-protein kinase 3, Mammalian STE20-like protein kinase 2, MST-2, STE20-like kinase MST2, Serine/threonine-protein kinase 3 36kDa subunit, MST2/N, Serine/threonine-protein kinase 3 20kDa subunit, MST2/C, Stk3, Mess1, Mst2

• Tested Applications:

  WB

• Physical Properties:

  Liquid