Mouse Irf3 Antibody

• Background:

  Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains (By similarity) .

• NCBI Gene ID:

  54131

• Swiss Prot:

  P70671

• Accession Number:

  P70671

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This Mouse Irf3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-72 amino acids from the N-terminal region of mouse Irf3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology, Inflammasomes

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Pig

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  47 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  Irf3

• NCBI Official Name:

  Interferon regulatory factor 3

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Marichal, T., et al. J. Allergy Clin. Immunol. 126 (4) :836-844 (2010)

• Background Reference 02:

  Menachery, V.D., et al. J. Virol. 84 (19) :9685-9694 (2010)

• Background Reference 03:

  Carrigan, S.O., et al. J. Immunol. 185 (6) :3602-3609 (2010)

• Background Reference 04:

  Wang, J., et al. J. Immunol. 185 (3) :1720-1729 (2010)

• Other Product Names:

  Interferon regulatory factor 3, IRF-3, Irf3

• Tested Applications:

  WB

• Physical Properties:

  Liquid