GCAP1 Antibody

• Background:

  Guanylate cyclase-activating protein is a l Ca (2+) -binding protein that upregulates synthesis of cGMP in photoreceptors. The known mammalian GCAPs are more than 90% similar, consisting of 201 to 205 amino acids, and containing 3 identically conserved Ca (2+) -binding sites. The GUCA1A gene, also termed GCAP1, is transcribed into a single 1.7-kb mRNA species detectable only in the retina. In a 4-generation British family with typical clinical features of autosomal dominant cone dystrophy a tyr99-to-cys mutation) in the GUCA1A gene has been identified. Another family with a pro50-to-leu mutation in GUCA1A demonstrated phenotypic variability ranging from mild photophobia to rod-cone dystrophy. The mutant protein could activate guanylate cyclase 1 (GUCY2D) and displayed similar calcium sensitivity to wildtype protein. However, there was a marked increase in the susceptibility to protease degradation and a reduction in the thermal stability of the pro50-to-leu mutation, which may depress cellular concentration and thereby contribute to retinal cell mortality.

• NCBI Gene ID:

  2978

• Swiss Prot:

  P43080

• Accession Number:

  P43080

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This GCAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-191 amino acids from the C-terminal region of human GCAP1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  23 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  GUCA1A

• NCBI Official Name:

  Guanylyl cyclase-activating protein 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Pennesi, M.E., et al., Proc. Natl. Acad. Sci. U.S.A. 100 (11) :6783-6788 (2003) .

• Background Reference 02:

  Payne, A.M., et al., Hum. Mol. Genet. 7 (2) :273-277 (1998) .

• Background Reference 03:

  Subbaraya, I., et al., J. Biol. Chem. 269 (49) :31080-31089 (1994) .

• Other Product Names:

  Guanylyl cyclase-activating protein 1, GCAP 1, Guanylate cyclase activator 1A, GUCA1A, C6orf131, GCAP, GCAP1, GUCA1

• Tested Applications:

  WB, IHC-P

• Protein ID:

  46577585

• Physical Properties:

  Liquid