SAP18 Antibody

• Background:

  Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2.

• NCBI Gene ID:

  10284

• Swiss Prot:

  O00422

• Accession Number:

  O00422

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This SAP18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 58-86 amino acids from the Central region of human SAP18.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  18 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  SAP18

• NCBI Official Name:

  Histone deacetylase complex subunit SAP18

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Sorin, M., et al. PLoS Pathog. (6), E1000463 (2009) :

• Background Reference 02:

  McCallum, S.A., et al. Biochemistry 45 (39) :11974-11982 (2006)

• Background Reference 03:

  Joselin, A.P., et al. J. Biol. Chem. 281 (18) :12475-12484 (2006)

• Background Reference 04:

  Schwerk, C., et al. Mol. Cell. Biol. 23 (8) :2981-2990 (2003)

• Other Product Names:

  Histone deacetylase complex subunit SAP18, 18 kDa Sin3-associated polypeptide, 2HOR0202, Cell growth-inhibiting gene 38 protein, Sin3-associated polypeptide p18, SAP18

• Tested Applications:

  WB

• Physical Properties:

  Liquid