WHSC2 Antibody

• Background :

  This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation.

• NCBI Gene ID :

  7469

• Swiss Prot :

  Q9H3P2

• Accession Number :

  Q9H3P2

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This WHSC2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-279 amino acids from the Central region of human WHSC2.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Other

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Homology :

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  57 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  NELFA

• NCBI Official Name :

  Negative elongation factor A

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Background Reference 02 :

  Narita, T., et al. Mol. Cell 26 (3) :349-365 (2007)

• Background Reference 03 :

  Olsen, J.V., et al. Cell 127 (3) :635-648 (2006)

• Background Reference 04 :

  Beausoleil, S.A., et al. Nat. Biotechnol. 24 (10) :1285-1292 (2006)

• Other Product Names :

  Negative elongation factor A, NELF-A, Wolf-Hirschhorn syndrome candidate 2 protein, NELFA, WHSC2

• Tested Applications :

  WB

• Protein ID :

  212276499

• Physical Properties :

  Liquid