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COBRA1 Antibody

CAT: 0223-57-973Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-57-973Size:400 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040) . NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]) .
NCBI Gene ID
25920
Swiss Prot
Q8WX92
Accession Number
Q8WX92
Host
Rabbit
Reactivity
Human
Immunogen
This COBRA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-285 amino acids from the Central region of human COBRA1.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Other
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Mouse
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
66 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
NELFB
NCBI Official Name
Negative elongation factor B
NCBI Organism
Homo sapiens
Background Reference 01
Sun, J., et al. J. Cell. Biochem. 103 (6) :1798-1807 (2008)
Background Reference 02
Sun, J., et al. J. Steroid Biochem. Mol. Biol. 107 (3-5), 131-139 (2007) :
Background Reference 03
Wu, C., et al. Proteomics 7 (11) :1775-1785 (2007)
Background Reference 04
Narita, T., et al. Mol. Cell 26 (3) :349-365 (2007)
Other Product Names
Negative elongation factor B, NELF-B, Cofactor of BRCA1, NELFB, COBRA1, KIAA1182
Tested Applications
WB
Protein ID
38372378
Physical Properties
Liquid

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