MED6 Antibody

• Background :

  Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

• NCBI Gene ID :

  10001

• Swiss Prot :

  O75586

• Accession Number :

  O75586

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This MED6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-164 amino acids from the Central region of human MED6.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Other

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  28 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  MED6

• NCBI Official Name :

  Mediator of RNA polymerase II transcription subunit 6

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Pavri, R., et al. Mol. Cell 18 (1) :83-96 (2005)

• Background Reference 02 :

  Sato, S., et al. Mol. Cell 14 (5) :685-691 (2004)

• Background Reference 03 :

  Yang, F., et al. Proc. Natl. Acad. Sci. U.S.A. 101 (8) :2339-2344 (2004)

• Background Reference 04 :

  Tomomori-Sato, C., et al. J. Biol. Chem. 279 (7) :5846-5851 (2004)

• Other Product Names :

  Mediator of RNA polymerase II transcription subunit 6, Activator-recruited cofactor 33 kDa component, ARC33, Mediator complex subunit 6, hMed6, Renal carcinoma antigen NY-REN-28, MED6, ARC33

• Tested Applications :

  WB

• Physical Properties :

  Liquid