TPX2 Antibody

• Background:

  TPX2 is a spindle assembly factor. Required for normal assembly of mitotic spindles. Required for normal assembly of microtubules during apoptosis. Required for chromatin and/or kinetochore dependent microtubule nucleation. Mediates AURKA localization to spindle microtubules. Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.

• NCBI Gene ID:

  22974

• Swiss Prot:

  Q9ULW0

• Accession Number:

  Q9ULW0

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This TPX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-448 amino acids from the Central region of human TPX2.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  86 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  TPX2

• NCBI Official Name:

  Targeting protein for Xklp2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Olson, J.E., et al. Breast Cancer Res. Treat. (2010) In press :

• Background Reference 02:

  Hosgood, H.D. III, et al. Occup Environ Med 66 (12) :848-853 (2009)

• Background Reference 03:

  Bibby, R.A., et al. J. Biol. Chem. 284 (48) :33177-33184 (2009)

• Background Reference 04:

  Shigeishi, H., et al. Int. J. Oncol. 34 (6) :1565-1571 (2009)

• Other Product Names:

  Targeting protein for Xklp2, Differentially expressed in cancerous and non-cancerous lung cells 2, DIL-2, Hepatocellular carcinoma-associated antigen 519, Hepatocellular carcinoma-associated antigen 90, Protein fls353, Restricted expression proliferation-associated protein 100, p100, TPX2, C20orf1, C20orf2, DIL2, HCA519

• Tested Applications:

  WB

• Protein ID:

  13124096

• Physical Properties:

  Liquid