NRF1 Antibody

• Background:

  This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for 'nuclear factor (erythroid-derived 2) -like 1' which has an official symbol of NFE2L1.

• NCBI Gene ID:

  4899

• Swiss Prot:

  Q16656

• Accession Number:

  Q16656

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This NRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 226-255 amino acids from the Central region of human NRF1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Zebrafish, Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  54 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  NRF1

• NCBI Official Name:

  Nuclear respiratory factor 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Gonen, N., et al. J. Biol. Chem. 285 (44) :33602-33613 (2010)

• Background Reference 02:

  Zhang, Y., et al. Biochem. J. 430 (3) :497-510 (2010)

• Background Reference 03:

  Shao, D., et al. Mitochondrion 10 (5) :516-527 (2010)

• Background Reference 04:

  Adam, T., et al. Biochem. Biophys. Res. Commun. 398 (3) :495-499 (2010)

• Other Product Names:

  Nuclear respiratory factor 1, NRF-1, Alpha palindromic-binding protein, Alpha-pal, NRF1

• Tested Applications:

  WB

• Protein ID:

  12643732

• Physical Properties:

  Liquid