ARFGAP3 Antibody

• Background:

  The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1) -bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene.

• NCBI Gene ID:

  26286

• Swiss Prot:

  Q9NP61

• Accession Number:

  Q9NP61

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This ARFGAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-473 amino acids from the C-terminal region of human ARFGAP3.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  57 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  ARFGAP3

• NCBI Official Name:

  ADP-ribosylation factor GTPase-activating protein 3

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Background Reference 02:

  Saitoh, A., et al. J. Biol. Chem. 284 (20) :13948-13957 (2009)

• Background Reference 03:

  Weimer, C., et al. J. Cell Biol. 183 (4) :725-735 (2008)

• Background Reference 04:

  Frigerio, G., et al. Traffic 8 (11) :1644-1655 (2007)

• Other Product Names:

  ADP-ribosylation factor GTPase-activating protein 3, ARF GAP 3, ARFGAP3, ARFGAP1

• Tested Applications:

  WB

• Protein ID:

  21263420

• Physical Properties:

  Liquid