RFXAP Antibody

• Background:

  Major histocompatibility (MHC) class II molecules are transmembrane proteins that have a central role in development and control of the immune system. The protein encoded by this gene, along with regulatory factor X-associated ankyrin-containing protein and regulatory factor-5, forms a complex that binds to the X box motif of certain MHC class II gene promoters and activates their transcription. Once bound to the promoter, this complex associates with the non-DNA-binding factor MHC class II transactivator, which controls the cell type specificity and inducibility of MHC class II gene expression. Mutations in this gene have been linked to bare lymphocyte syndrome type II, complementation group D. Transcript variants utilizing different polyA signals have been found for this gene.

• NCBI Gene ID:

  5994

• Swiss Prot:

  O00287

• Accession Number:

  O00287

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This RFXAP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 196-225 amino acids from the C-terminal region of human RFXAP.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  28 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  RFXAP

• NCBI Official Name:

  Regulatory factor X-associated protein

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Laird, K.M., et al. J. Mol. Biol. 403 (1) :40-51 (2010)

• Background Reference 02:

  Bushway, M., et al. Immunogenetics 62 (2) :109-116 (2010)

• Background Reference 03:

  Briggs, L., et al. Proteins 76 (3) :655-664 (2009)

• Background Reference 04:

  Garvie, C.W., et al. Biochim. Biophys. Acta 1779 (12) :797-804 (2008)

• Other Product Names:

  Regulatory factor X-associated protein, RFX-associated protein, RFX DNA-binding complex 36 kDa subunit, RFXAP

• Tested Applications:

  WB

• Physical Properties:

  Liquid