USP20 Antibody

• Background:

  Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2) . Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains.

• NCBI Gene ID:

  10868

• Swiss Prot:

  Q9Y2K6

• Accession Number:

  Q9Y2K6

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This USP20 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 310-339 amino acids from the Central region of human USP20.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  102 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  USP20

• NCBI Official Name:

  Ubiquitin carboxyl-terminal hydrolase 20

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Yoshida, T., et al. Int. J. Mol. Med. 25 (4) :649-656 (2010)

• Background Reference 02:

  Oguri, M., et al. Am. J. Hypertens. 23 (1) :70-77 (2010)

• Background Reference 03:

  Berthouze, M., et al. EMBO J. 28 (12) :1684-1696 (2009)

• Background Reference 04:

  Olsen, J.V., et al. Cell 127 (3) :635-648 (2006)

• Other Product Names:

  Ubiquitin carboxyl-terminal hydrolase 20, Deubiquitinating enzyme 20, Ubiquitin thioesterase 20, Ubiquitin-specific-processing protease 20, VHL-interacting deubiquitinating enzyme 2, hVDU2, USP20, KIAA1003, LSFR3A, VDU2

• Tested Applications:

  WB

• Protein ID:

  116242837

• Physical Properties:

  Liquid