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TDT Antibody

CAT: 0223-57-757Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-57-757Size:400 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
This gene is a member of the DNA polymerase type-X family and encodes a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. In vivo, the encoded protein is expressed in a restricted population of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
NCBI Gene ID
1791
Swiss Prot
P04053
Accession Number
P04053
Host
Rabbit
Reactivity
Human
Immunogen
This TDT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-70 amino acids from the N-terminal region of human TDT.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Immunology
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
59 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
DNTT
NCBI Official Name
DNA nucleotidylexotransferase
NCBI Organism
Homo sapiens
Background Reference 01
Kubota, T., et al. Genes Cells 12 (8) :941-959 (2007)
Background Reference 02
O'Malley, D.P., et al. Haematologica 91 (8) :1139-1140 (2006)
Background Reference 03
Grupe, A., et al. Am. J. Hum. Genet. 78 (1) :78-88 (2006)
Background Reference 04
Thai, T.H., et al. J. Immunol. 173 (6) :4009-4019 (2004)
Other Product Names
DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase, DNTT, TDT
Tested Applications
WB
Protein ID
311033533
Physical Properties
Liquid

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