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CHFR Antibody

CAT: 0223-57-729Size: 400 µLDry Ice: NoHazardous: No
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CAT#:0223-57-729Size:400 µL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.
NCBI Gene ID
55743
Swiss Prot
Q96EP1
Accession Number
Q96EP1
Host
Rabbit
Reactivity
Human
Immunogen
This CHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-504 amino acids from the C-terminal region of human CHFR.
Clonality
Polyclonal
Conjugation
Unconjugated
Type
Primary Antibodies
Field of Research
Cancer, Cell Cycle
Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Batch dependent
Homology
Predicted species reactivity based on immunogen sequence: Mouse
Buffer
Supplied in PBS with 0.09% (W/V) sodium azide.
Modification
None
Shipping Conditions
Blue Ice
Storage Conditions
Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Calculated Molecular Weight
73 kDa
Fragment
Rabbit Ig
Applications Notes
For WB starting dilution is: 1:1000
Symbol
CHFR
NCBI Official Name
E3 ubiquitin-protein ligase CHFR
NCBI Organism
Homo sapiens
Background Reference 01
Soutto, M., et al. Cancer 116 (17) :4033-4042 (2010)
Background Reference 02
Kim, J.M., et al. Biochem. Biophys. Res. Commun. 395 (4) :515-520 (2010)
Background Reference 03
Hiraki, M., et al. World J. Gastroenterol. 16 (3) :330-338 (2010)
Background Reference 04
Baba, S., et al. Oncol. Rep. 22 (5) :1173-1179 (2009)
Other Product Names
E3 ubiquitin-protein ligase CHFR, 632-, Checkpoint with forkhead and RING finger domains protein, RING finger protein 196, CHFR, RNF196
Tested Applications
WB
Protein ID
41688511
Physical Properties
Liquid

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