CHFR Antibody

• Background:

  E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1. Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress.

• NCBI Gene ID:

  55743

• Swiss Prot:

  Q96EP1

• Accession Number:

  Q96EP1

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This CHFR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-504 amino acids from the C-terminal region of human CHFR.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  73 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  CHFR

• NCBI Official Name:

  E3 ubiquitin-protein ligase CHFR

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Soutto, M., et al. Cancer 116 (17) :4033-4042 (2010)

• Background Reference 02:

  Kim, J.M., et al. Biochem. Biophys. Res. Commun. 395 (4) :515-520 (2010)

• Background Reference 03:

  Hiraki, M., et al. World J. Gastroenterol. 16 (3) :330-338 (2010)

• Background Reference 04:

  Baba, S., et al. Oncol. Rep. 22 (5) :1173-1179 (2009)

• Other Product Names:

  E3 ubiquitin-protein ligase CHFR, 632-, Checkpoint with forkhead and RING finger domains protein, RING finger protein 196, CHFR, RNF196

• Tested Applications:

  WB

• Protein ID:

  41688511

• Physical Properties:

  Liquid