Mouse Rps6kb1 Antibody

Rps6kb1 acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-136' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-136'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates POLDIP3 (By similarity) .

72508

Q8BSK8

Q8BSK8

Human, Mouse

This Mouse Rps6kb1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 481-509 amino acids from the C-terminal region of mouse Rps6kb1.

Unconjugated

Primary Antibodies

Cancer, Cell Cycle, Signal Transduction

This antibody is purified through a protein A column, followed by peptide affinity purification.

Batch dependent

Predicted species reactivity based on immunogen sequence: Bovine, Rabbit, Rat

Supplied in PBS with 0.09% (W/V) sodium azide.

None

Blue Ice

Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

59 kDa

Rabbit Ig

For WB starting dilution is: 1:1000

Rps6kb1

Ribosomal protein S6 kinase beta-1

Mus musculus

Lai, K.P., et al. EMBO J. 29 (17) :2994-3006 (2010)

Michels, A.A., et al. Mol. Cell. Biol. 30 (15) :3749-3757 (2010)

Witkowski, S., et al. FEBS Lett. 584 (13) :2891-2895 (2010)

Carnevalli, L.S., et al. Dev. Cell 18 (5) :763-774 (2010)

Ribosomal protein S6 kinase beta-1, S6K-beta-1, S6K1, 70 kDa ribosomal protein S6 kinase 1, P70S6K1, p70-S6K 1, Ribosomal protein S6 kinase I, S6K, p70 ribosomal S6 kinase alpha, p70 S6 kinase alpha, p70 S6K-alpha, p70 S6KA, Rps6kb1

WB