Mouse Ern1 Antibody

• Background:

  Ern1 senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

• NCBI Gene ID:

  78943

• Swiss Prot:

  Q9EQY0

• Accession Number:

  Q9EQY0

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This Mouse Ern1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 827-855 amino acids from the C-terminal region of mouse Ern1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  110 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  Ern1

• NCBI Official Name:

  Serine/threonine-protein kinase/endoribonuclease IRE1

• NCBI Organism:

  Mus musculus

• Background Reference 01:

  Li, H., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (37) :16113-16118 (2010)

• Background Reference 02:

  Auf, G., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (35) :15553-15558 (2010)

• Background Reference 03:

  Oikawa, D., et al. FEBS Lett. 584 (5) :1066-1070 (2010)

• Background Reference 04:

  Yang, L., et al. PLoS ONE (7), E11621 (2010) :

• Other Product Names:

  Serine/threonine-protein kinase/endoribonuclease IRE1, Endoplasmic reticulum-to-nucleus signaling 1, Inositol-requiring protein 1, Ire1-alpha, IRE1a, Serine/threonine-protein kinase, Endoribonuclease, 3126-, Ern1 {ECO:0000312|MGI:MGI:1930134}

• Tested Applications:

  WB

• Physical Properties:

  Liquid