IBSP Antibody

• Background:

  The protein encoded by this gene is a major structural protein of the bone matrix. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor.

• NCBI Gene ID:

  3381

• Swiss Prot:

  P21815

• Accession Number:

  P21815

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This IBSP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-99 amino acids from the N-terminal region of human IBSP.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  35 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  IBSP

• NCBI Official Name:

  Bone sialoprotein 2

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Giroux, S., et al. Bone 47 (5) :975-981 (2010)

• Background Reference 02:

  Koller, D.L., et al. J. Clin. Endocrinol. Metab. 95 (4) :1802-1809 (2010)

• Background Reference 03:

  Yerges, L.M., et al. J. Bone Miner. Res. 24 (12) :2039-2049 (2009)

• Background Reference 04:

  Gordon, J.A., et al. J. Cell. Biochem. 107 (6) :1118-1128 (2009)

• Other Product Names:

  Bone sialoprotein 2, Bone sialoprotein II, BSP II, Cell-binding sialoprotein, Integrin-binding sialoprotein, IBSP, BNSP

• Tested Applications:

  WB

• Protein ID:

  317373545

• Physical Properties:

  Liquid