ACK1 Antibody

• Background:

  This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP) -stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

• NCBI Gene ID:

  10188

• Swiss Prot:

  Q07912

• Accession Number:

  Q07912

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-290 amino acids from the Central region of human ACK1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  115 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  TNK2

• NCBI Official Name:

  Activated CDC42 kinase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Mahajan, K., et al. J. Cell. Physiol. 224 (2) :327-333 (2010)

• Background Reference 02:

  Chua, B.T., et al. Mol Oncol 4 (4) :323-334 (2010)

• Background Reference 03:

  Prieto-Echague, V., et al. J. Biol. Chem. 285 (14) :10605-10615 (2010)

• Background Reference 04:

  Lin, Q., et al. Mol. Cell. Biol. 30 (6) :1541-1554 (2010)

• Other Product Names:

  Activated CDC42 kinase 1, ACK-1, Tyrosine kinase non-receptor protein 2, TNK2, ACK1

• Tested Applications:

  WB

• Protein ID:

  229462980

• Physical Properties:

  Liquid