ACK1 Antibody

• Background :

  This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP) -stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.

• NCBI Gene ID :

  10188

• Swiss Prot :

  Q07912

• Accession Number :

  Q07912

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This ACK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-290 amino acids from the Central region of human ACK1.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cancer, Signal Transduction

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Homology :

  Predicted species reactivity based on immunogen sequence: Bovine, Mouse

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  115 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  TNK2

• NCBI Official Name :

  Activated CDC42 kinase 1

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Mahajan, K., et al. J. Cell. Physiol. 224 (2) :327-333 (2010)

• Background Reference 02 :

  Chua, B.T., et al. Mol Oncol 4 (4) :323-334 (2010)

• Background Reference 03 :

  Prieto-Echague, V., et al. J. Biol. Chem. 285 (14) :10605-10615 (2010)

• Background Reference 04 :

  Lin, Q., et al. Mol. Cell. Biol. 30 (6) :1541-1554 (2010)

• Other Product Names :

  Activated CDC42 kinase 1, ACK-1, Tyrosine kinase non-receptor protein 2, TNK2, ACK1

• Tested Applications :

  WB

• Protein ID :

  229462980

• Physical Properties :

  Liquid