VPS4B Antibody

• Background:

  The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. Mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be a yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 18; the gene for the other resides on chromosome 16. [provided by RefSeq].

• NCBI Gene ID:

  9525

• Swiss Prot:

  O75351

• Accession Number:

  O75351

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This VPS4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-332 amino acids from the C-terminal region of human VPS4B.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  49 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  VPS4B

• NCBI Official Name:

  Vacuolar protein sorting-associated protein 4B

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Morita, E., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (29) :12889-12894 (2010)

• Background Reference 02:

  McDonough, C.W., et al. Hum. Genet. (2009) In press :

• Background Reference 03:

  Bruce, E.A., et al. Virology 390 (2) :268-278 (2009)

• Background Reference 04:

  Inoue, M., et al. Traffic 9 (12) :2180-2189 (2008)

• Other Product Names:

  Vacuolar protein sorting-associated protein 4B, Cell migration-inducing gene 1 protein, Suppressor of K (+) transport growth defect 1, Protein SKD1, VPS4B, SKD1, VPS42

• Tested Applications:

  WB

• Physical Properties:

  Liquid