NFE2 Antibody

• Background :

  Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR) . This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

• NCBI Gene ID :

  4778

• Swiss Prot :

  Q16621

• Accession Number :

  Q16621

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This NFE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 312-340 amino acids from the C-terminal region of human NFE2.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Other

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  41 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  NFE2

• NCBI Official Name :

  Transcription factor NF-E2 45 kDa subunit

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Wang, W., et al. Blood 116 (2) :254-266 (2010)

• Background Reference 02 :

  Zhou, Z., et al. J. Biol. Chem. 285 (21) :15894-15905 (2010)

• Background Reference 03 :

  Motohashi, H., et al. Blood 115 (3) :677-686 (2010)

• Background Reference 04 :

  Lee, T.L., et al. Proc. Natl. Acad. Sci. U.S.A. 107 (1) :52-57 (2010)

• Other Product Names :

  Transcription factor NF-E2 45 kDa subunit, Leucine zipper protein NF-E2, Nuclear factor, erythroid-derived 2 45 kDa subunit, p45 NF-E2, NFE2

• Tested Applications :

  WB

• Protein ID :

  6831585

• Physical Properties :

  Liquid