EIF2S1 Antibody

• Background:

  The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161) . The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]) .

• NCBI Gene ID:

  1965

• Swiss Prot:

  P05198

• Accession Number:

  P05198

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  This EIF2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human EIF2S1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Rat, Chicken, Yeast, Bovine, Drosophila

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  36 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  EIF2S1

• NCBI Official Name:

  Eukaryotic translation initiation factor 2 subunit 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Shiota, M., et al. Int. J. Oncol. 36 (6) :1521-1531 (2010)

• Background Reference 02:

  Kulkarni, A.P., et al. Indian J. Biochem. Biophys. 47 (2) :67-74 (2010)

• Background Reference 03:

  Wehner, K.A., et al. Mol. Cell. Biol. 30 (8) :2006-2016 (2010)

• Background Reference 04:

  Backes, S., et al. J. Gen. Virol. 91 (PT 2), 470-482 (2010) :

• Other Product Names:

  Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2S1, EIF2A

• Tested Applications:

  WB, IHC-P, IF

• Protein ID:

  124200

• Physical Properties:

  Liquid