AR Antibody

• Background:

  The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract causes spinal bulbar muscular atrophy (Kennedy disease) . Mutations in this gene are also associated with complete androgen insensitivity (CAIS) . Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq].

• NCBI Gene ID:

  367

• Swiss Prot:

  P10275

• Accession Number:

  P10275

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This AR antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-309 amino acids from the Central region of human AR.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Monkey

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  99 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  AR

• NCBI Official Name:

  Androgen receptor

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Shu, S.K., et al. J. Biol. Chem. 285 (43) :33045-33053 (2010)

• Background Reference 02:

  Nedelsky, N.B., et al. Neuron 67 (6) :936-952 (2010)

• Background Reference 03:

  Panda, B., et al. Gynecol. Endocrinol. (2010) In press :

• Background Reference 04:

  Schneider, G., et al. AmGeriatr Psychiatry (2010) In press :

• Other Product Names:

  Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4, AR, DHTR, NR3C4

• Tested Applications:

  WB

• Protein ID:

  113830

• Physical Properties:

  Liquid