SPAG8 Antibody

• Background :

  The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.

• NCBI Gene ID :

  26206

• Swiss Prot :

  Q99932

• Accession Number :

  Q99932

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  This SPAG8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-411 amino acids from the C-terminal region of human SPAG8.

• Clonality :

  Polyclonal

• Conjugation :

  Unconjugated

• Type :

  Primary Antibodies

• Field of Research :

  Cell Cycle, Signal Transduction

• Purification :

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration :

  Batch dependent

• Buffer :

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification :

  None

• Shipping Conditions :

  Blue Ice

• Storage Conditions :

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight :

  45 kDa

• Fragment :

  Rabbit Ig

• Applications Notes :

  For WB starting dilution is: 1:1000

• Symbol :

  SPAG8

• NCBI Official Name :

  Sperm-associated antigen 8

• NCBI Organism :

  Homo sapiens

• Background Reference 01 :

  Wu, H., et al. FEBS Lett. 584 (13) :2807-2815 (2010)

• Background Reference 02 :

  Li, R., et al. Cell Biochem. Funct. 27 (5) :264-268 (2009)

• Background Reference 03 :

  Cheng, G.Y., et al. Asian J. Androl. 9 (1) :23-29 (2007)

• Background Reference 04 :

  Tang, X., et al. J. Mol. Med. 82 (6) :383-388 (2004)

• Other Product Names :

  Sperm-associated antigen 8, HSD-1, Sperm membrane protein 1, SMP-1, Sperm membrane protein BS-84, SPAG8

• Tested Applications :

  WB

• Protein ID :

  62511128

• Physical Properties :

  Liquid