APEH Antibody

• Background:

  APEH is the enzyme acylpeptide hydrolase, which catalyzes the hydrolysis of the terminal acetylated amino acid preferentially from small acetylated peptides. The acetyl amino acid formed by this hydrolase is further processed to acetate and a free amino acid by an aminoacylase. APEH gene is located within the same region of chromosome 3 (3p21) as the aminoacylase gene, and deletions at this locus are also associated with a decrease in aminoacylase activity. The acylpeptide hydrolase is a homotetrameric protein of 300 kDa with each subunit consisting of 732 amino acid residues. The protein can play an important role in destroying oxidatively damaged proteins in living cells.

• NCBI Gene ID:

  327

• Swiss Prot:

  P13798

• Accession Number:

  P13798

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This APEH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 174-204 amino acids from the N-terminal region of human APEH.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  81 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  APEH

• NCBI Official Name:

  Acylamino-acid-releasing enzyme

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Perrier, J., Giardina, T. Biol. Cell 94 (1), 45-54 (2002)

• Background Reference 02:

  Erlandsson, R., Boldog, F. Oncogene (7), 1293-1295 (1991)

• Background Reference 03:

  Erlandsson, R., Bergerheim, U.S. Oncogene (8), 1207-1211 (1990)

• Other Product Names:

  Acylamino-acid-releasing enzyme, AARE, Acyl-peptide hydrolase, APH, Acylaminoacyl-peptidase, Oxidized protein hydrolase, OPH, APEH, D3F15S2, D3S48E, DNF15S2

• Tested Applications:

  WB

• Protein ID:

  38258902

• Physical Properties:

  Liquid