UBTF Antibody

• Background:

  Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs') . Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]) . UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]) .

• NCBI Gene ID:

  7343

• Swiss Prot:

  P17480

• Accession Number:

  P17480

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This UBTF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 631-660 amino acids from the C-terminal region of human UBTF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Other

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  89 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  UBTF

• NCBI Official Name:

  Nucleolar transcription factor 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Hisaoka, M., et al. Mol. Cell. Biol. 30 (20) :4952-4964 (2010)

• Background Reference 02:

  Liu, Z., et al. Proteins 77 (2) :432-447 (2009)

• Background Reference 03:

  Lin, C.I., et al. Biochem. Biophys. Res. Commun. 386 (2) :396-401 (2009)

• Background Reference 04:

  Grob, A., et al. J. Cell. Sci. 122 (PT 4), 489-498 (2009) :

• Other Product Names:

  Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1, UBF-1, UBTF, UBF, UBF1

• Tested Applications:

  WB

• Protein ID:

  136652

• Physical Properties:

  Liquid