ATP5J Antibody

• Background:

  Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8) . This gene encodes the F6 subunit of the Fo complex, required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.

• NCBI Gene ID:

  522

• Swiss Prot:

  P18859

• Accession Number:

  P18859

• Host:

  Rabbit

• Reactivity:

  Mouse, Rat

• Immunogen:

  This ATP5J antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from the Central region of human ATP5J.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Obesity, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Monkey

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  13 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  ATP5J

• NCBI Official Name:

  ATP synthase-coupling factor 6, mitochondrial

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Osanai, T., et al. Cardiovasc. Res. 81 (4) :780-787 (2009)

• Background Reference 02:

  Wang, L., et al. Cancer Epidemiol. Biomarkers Prev. 17 (12) :3558-3566 (2008)

• Background Reference 03:

  Kumagai, A., et al. Atherosclerosis 200 (1) :45-50 (2008)

• Background Reference 04:

  Chai, S.B., et al. Circ. J. 71 (5) :693-697 (2007)

• Other Product Names:

  ATP synthase-coupling factor 6, mitochondrial, ATPase subunit F6, ATP5J, ATP5A, ATPM

• Tested Applications:

  WB, IHC-P, IF

• Protein ID:

  114688

• Physical Properties:

  Liquid