PCAF Antibody

• Background:

  CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

• NCBI Gene ID:

  8850

• Swiss Prot:

  Q92831

• Accession Number:

  Q92831

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This PCAF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-283 amino acids from the N-terminal region of human PCAF.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cell Cycle

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Mouse

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  93 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  KAT2B

• NCBI Official Name:

  Histone acetyltransferase KAT2B

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Perez, R.E., et al. J. Cell. Physiol. 225 (2) :394-405 (2010)

• Background Reference 02:

  Mooney, S.M., et al. J. Biol. Chem. 285 (40) :30443-30452 (2010)

• Background Reference 03:

  Aoyama, T., et al. J. Biol. Chem. 285 (39) :29842-29850 (2010)

• Background Reference 04:

  Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :

• Other Product Names:

  Histone acetyltransferase KAT2B, Histone acetyltransferase PCAF, Histone acetylase PCAF, Lysine acetyltransferase 2B, P300/CBP-associated factor, P/CAF, KAT2B, PCAF

• Tested Applications:

  WB

• Protein ID:

  83287776

• Physical Properties:

  Liquid