HRAS Antibody

• Background:

  This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

• NCBI Gene ID:

  3265

• Swiss Prot:

  P01112

• Accession Number:

  P01112

• Host:

  Rabbit

• Reactivity:

  Human, Mouse

• Immunogen:

  This HRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-176 amino acids from the C-terminal region of human HRAS.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Cancer, Cell Cycle, Signal Transduction

• Purification:

  This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis

• Concentration:

  Batch dependent

• Homology:

  Predicted species reactivity based on immunogen sequence: Chicken, Rat

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  21 kDa

• Fragment:

  Rabbit Ig

• Symbol:

  HRAS

• NCBI Official Name:

  GTPase HRas

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Ma, Z., et al. Oncogene 29 (41) :5559-5567 (2010)

• Background Reference 02:

  Van Engen-van Grunsven, A.C., et al. Am. J. Surg. Pathol. 34 (10) :1436-1441 (2010)

• Background Reference 03:

  Li, H., et al. Oncogene 29 (36) :5083-5094 (2010)

• Background Reference 04:

  Kwack, K.B., et al. KoreanGastroenterol 56 (2) :78-82 (2010)

• Other Product Names:

  GTPase HRas, H-Ras-1, Ha-Ras, Transforming protein p21, c-H-ras, p21ras, GTPase HRas, N-terminally processed, HRAS, HRAS1

• Tested Applications:

  WB, IF

• Protein ID:

  131869

• Physical Properties:

  Liquid