GAD1 Antibody

• Background:

  This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

• NCBI Gene ID:

  2571

• Swiss Prot:

  Q99259

• Accession Number:

  Q99259

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  This GAD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 514-543 amino acids from the C-terminal region of human GAD1.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Immunology, Obesity, Neuroscience, Signal Transduction

• Purification:

  This antibody is purified through a protein A column, followed by peptide affinity purification.

• Concentration:

  Batch dependent

• Buffer:

  Supplied in PBS with 0.09% (W/V) sodium azide.

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

• Calculated Molecular Weight:

  67 kDa

• Fragment:

  Rabbit Ig

• Applications Notes:

  For WB starting dilution is: 1:1000

• Symbol:

  GAD1

• NCBI Official Name:

  Glutamate decarboxylase 1

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Lanoue, A.C., et al. Exp. Neurol. 226 (1) :207-217 (2010)

• Background Reference 02:

  Jia, P., et al. Schizophr. Res. 122 (1-3), 38-42 (2010) :

• Background Reference 03:

  Terranova, C., et al. Alcohol 44 (5) :407-413 (2010)

• Background Reference 04:

  Ruano, G., et al. Pharmacogenomics 11 (7) :959-971 (2010)

• Other Product Names:

  Glutamate decarboxylase 1, 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 67 kDa isoform, GAD1, GAD, GAD67

• Tested Applications:

  WB

• Protein ID:

  1352213

• Physical Properties:

  Liquid