Choline Acetyltransferase Antibody

• Background:

  Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999) . Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993) . It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999) .

• NCBI Gene ID:

  1103

• Swiss Prot:

  P28329

• Accession Number:

  P28329

• Host:

  Goat

• Reactivity:

  Human, Mouse, Rat, Goat, Chicken

• Immunogen:

  Native choline acetyltransferase purified from human placenta.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Affinity Purified

• Concentration:

  Batch dependent

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Choline Acetyltransferase antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

• Calculated Molecular Weight:

  70

• Specificity:

  Specific for the ~ 70k choline acetyltransferase protein

• Symbol:

  CHAT

• NCBI Official Name:

  Choline O-acetyltransferase

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Karson CN, Casanova MF, Kleinman JE, Griffin WS (1993) Choline acetyltransferase in schizophrenia. Am. J.Psychiatry 150:454-459.

• Background Reference 02:

  Holt DJ, Herman MM, Hyde TM, Kleinman JE, Cinton CM, German DC, Hersh LB, Greybiel AM, Saper CB (1999) Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience 94 (1) :21-31.

• Background Reference 03:

  Baskin DS, Browning JL, Pirozzolo FJ, Korporaal S, Baskin JA, Appel SH (1999) Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123.

• Tested Applications:

  WB, IHC

• Protein ID:

  281185509

• Physical Properties:

  Liquid