Neurofilament NF-L Antibody

• Background:

  Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68 kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease.

• NCBI Gene ID:

  4747

• Swiss Prot:

  P07196

• Accession Number:

  P07196

• Host:

  Chicken

• Reactivity:

  Human, Mouse, Rat, Bovine, Chicken

• Immunogen:

  Preparation of bovine spinal cord intermediate filaments

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Neuroscience

• Purification:

  Total IgY fraction

• Concentration:

  Batch dependent

• Modification:

  None

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Neurofilament NF-L antibody can be stored at -20˚ C and is stable at -20˚ C for at least 1 year.

• Calculated Molecular Weight:

  68

• Specificity:

  Specific for the ~68k Neurofilament L protein in Western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.

• Symbol:

  NEFL

• NCBI Official Name:

  Neurofilament, light polypeptide

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.

• Background Reference 02:

  Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46.

• Tested Applications:

  WB, IHC

• Protein ID:

  62511894

• Physical Properties:

  Liquid